qPCR Newsletter - April 2008
Dear researcher,
dear Gene Quantification page reader,
Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:
- update - High Resolution Melt (HRM)
- update - quantification via real-time PCR
- qPCR Symposium 2008
- European Seminar tour
- qPCR application workshops in 2008
------------
High Resolution Melting (HRM)
http://HRM.gene-
HRM is a novel, homogeneous, close-tube, post-PCR method, enabling
genomic researchers to analyze genetic variations (SNPs, mutations,
methylations) in PCR amplicons. It goes beyond the power of classical
melting curve analysis by allowing to study the thermal denaturation
of a double-stranded DNA in much more detail and with much higher
information yield than ever before. HRM characterizes nucleic acid
samples based on their disassociation (melting) behavior. Samples can
be discriminated according to their sequence, length, GC content or
strand complementarity. Even single base changes such as SNPs (single
nucleotide polymorphisms) can be readily identified.
The most important High Resolution Melting application is gene
scanning - the search for the presence of unknown variations in PCR
amplicons prior to or as an alternative to sequencing. Mutations in
PCR products are detectable by High Resolution Melting because they
change the shape of DNA melting curves. A combination of
new-generation DNA dyes, high-end instrumentation and sophisticated
analysis software allows to detect these changes and to derive
information about the underlying sequence constellation.
UPDATE - A lot new publications were added to the HRM pages =>
http://HRM.gene-
------------
real-time PCR - A technique enabling fast, quantitative and reliable
results
http://qPCR.
Some of the limitations of end-point detection in PCR have been
assuaged in real-time PCR systems, various are now on the market.
These systems offer many general technical advantages, including
reduced probabilities of variability and contamination, as well as
online monitoring and the lack of need for post reaction analyses.
Further, some of these systems were developed with contemporary
applications such as quantitative PCR, multiplexing, and
high-throughput analysis in mind.
UPDATE - Alot of basic papers are presented and new publicatiojns were
added to this section => http://qpcr.
------------
qPCR Symposium USA
10. - 13. November 2008
Clarion Hotel San Francisco Airport, Millbrae, CA , USA
http://www.gene-
High throughput platforms
High throughput applications, real-time RT-PCR arrays, digital PCR
Forthcoming technologies
Immuno PCR, Methylation sensitive PCR, SNP analysis, High resolution
melt, microRNA detection, Multiplex technologies
Single-cell qPCR
Pre-amplification, sub-cellular PCR, Expression heterogeneity, laser
microdissection, FACS sorting, Enrichment of rare cells
Multimarker diagnostics
Disease markers, Tissue specific markers, Cancer markers, Stem cells,
Differentiation markers, Cancer stem cells
Real-time PCR Expression Profiling
multivariate and multiway expression profiling, temporal expression
profiling, spatiotemporal maps
Pre-analytical Steps
Sampling technologies, Extraction methods, Reverse Transcription,
Quality Control, Standards, Standard Operating Procedures,
Interlaboratory Exercises
Normalization & Standardization
Normalization strategies, Reference genes, Spikes, Standard
curves,multiplexing
mRNA degradation
Data management and data treatment
software applications, data mining, data visualization, biostatistics,
multivariate statistics
Download TOPICS Flyer =>
http://www.gene-
Download SPEAKERS Flyer =>
http://www.gene-
------------
With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=> Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory
http://infoportal.
------------
Upcoming Events World-wide academic and commercial qPCR Events
http://events.
Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, ...
Please submit your qPCR event here => events@gene-
------------
WORKSHOP
TATAA Biocenter Germany - qPCR Application workshops
At the TATAA Biocenter Germany we offer qPCR application workshops,
the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses
are held in regularly in Göteborg, Sweden, in English and in
Freising-Weihenstep
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide. TATAA
Biocenter Germany courses are held in cooperation with the Institute
of Physiology, located at the Technical University of Munich, in
Freising-Weihenstep
(MUC). For more information and to register for the qPCR application
workshops, please see our web page:
http://tataa.
Course Occasions 2008:
3-day qPCR Core Module (Mon. - Wed.) and 2-day BioStatistics
Module (Thu. - Fri.)
* 5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH
gehalten, German language)
* 7 - 11th July 2008 (in Freising, Germany, English language)
Please register here => http://www.tataa.
------------
Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !
Best regards,
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.gene-
------------
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please use following LINK:
http://qPCRnews.
The qPCR NEWS and the Gene Quantification Pages are educational sites
with the only purpose of facilitating access to qPCR related
information on the internet. The qPCR NEWS and the Gene
Quantification Pages are edited by Michael W. Pfaffl and powered by
BioScience Events. Copyright © 2005 - 2008 All rights reserved. Any
unauthorized use, reproduction, or transfer of this message or its
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Cord Blood Registry
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The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
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